席建忠 教授

研究领域: 大规模功能基因筛选及药物研发
电话:
联系邮箱: jzxi@ pku.edu. cn

个人简历

国家杰出青年基金获得者

男，1974 年出生。1996 年毕业于北京理工大学化工与材料学院，2000 年在清华大学生物系获得细胞与分子生物学硕士，2004 年在加州大学洛杉矶分校获得生物医学工程专业博士学位。2005 年，加入北京大学工学院生物医学工程系，现任北京大学工学院研究员，博士生导师。目前担任中国化学会化学生物学专业委员会委员、中国医药生物技术协会生物芯片分会常务委员、中国生物医学工程学会生物医学测量分会委员等职务。主要从事大规模功能基因技术的研发和应用，包括细胞芯片、大规模药物筛选、基因操纵、miRNA、TALE 及CRISPR 技术开发和应用等。近五年，围绕药物筛选、药效评估动物模型等方面，取得了突出成绩，在 Nature, Nat. Biotechnol., Nat. Cell Biol., Nat. Commun., Proc. Natl. Acad. Sci. USA, Angew. Chem. Int. Ed.等专业杂志发表 30 余篇高水平学术论文；主持或承担过国家自然科学基金 4 项，“973 计划”课题 2 项及863 课题2 项。

主要研究领域

1.细胞芯片

2.大规模功能基因和药物 

3.靶向基因编辑2.药物动力学/药效动力学

代表性论文

1. Dou L, Meng X, Sui X, Wang S, Shen T, Huang X, Guo J, Fang W, Man Y, Xi J*, Li J*, MiR-19a regulates PTEN expression to mediate glycogen synthesis in hepatocytes. Sci Rep. (Accept)

2. Dou L, Wang S, Sui X, Meng X, Shen T, Huang X, Guo J, Fang W, Man Y, Xi J*, Li J*, MiR-301a Mediates the Effect of IL-6 on the AKT/GSK Pathway and Hepatic Glycogenesis by Regulating PTEN Expression. Cell Physiol Biochem 35:1413-1424,  2015

3. Cui C, Wang Y, Yang K, Wang Y, Yang J, Xi J, Zhao M, Wu J, Peng S, Preparation and Characterization of RGDS/Nanodiamond as a Vector for VEGF-siRNA Delivery. Journal of Biomedical Nanotechnology, 11:1, 70-80, 2015

4. Zhang Y, Sun J, Zou Y, Chen W, Zhang W, Xi JJ, Jiang X*, Barcoded microchips for biomolecular assays, Anal Chem. 87(2):900-6, 2015

5. Haisong Liu, Huan Yang, Dicong Zhu, Xin Sui, Juan Li, Zhen Liang, Lei Xu, Zeyu Chen, Anzhi Yao, Long Zhang, Xi Zhang, Xing Yi, Meng Liu, Shiqing Xu, Wenjian Zhang, Hua Lin, Lan Xie, Jinning Lou, Yong Zhang, Jianzhong Xi *, Hongkui Deng*, Systematically labeling developmental stage-specific genes for the study of pancreatic β-cell differentiation from human embryonic stem cells, Cell research 20: 1181-1200, 2014

6. Sun C, Fan Y, Li J, Wang G, Zhang H*, Xi JJ*, Efficient production of a gene mutant cell line through integrating TALENs and high-throughput cell cloning. J Lab Autom. 20(1):46-50, 2015

7. Zhang H, Li J, Hou S, Wang G, Jiang M, Sun C, Hu X, Zhuang F, Dai J*, Xi J*, Engineered TAL Effector Modulators for the Large-scale Gain-of-Function Screening, Nucleic Acid Research 42(14):e114, 2014

8. Hao Y, Yang J, Yin S, Zhang H, Fan Y, Sun C and Xi J*. The Synergistic Regulation of VEGF-mediated Angiogenesis Through miR-190 and Target Genes, RNA, 20, 1-9, 2014

9. Xu H, Yang J, Gao W, Li L, Li P, Zhang L, Gong Y-N, Peng X, Xi J, Chen S, Wang F & Shao F, Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome, Nature 513(7517):237-41, 2014

10. Yao J, Huang J, Hai T, Wang X, Qin G, Zhang H, Wu R, Cao C, Xi JJ, Yuan Z, Zhao J, Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs. Sci Rep. 4:6926, 2014

11. Fan Y, Yin S, Hao Y. Yang J, Zhang H, Sun C, Ma M, Chang Q, and Xi, J*. miR-19b promotes tumor growth and metastasis via targeting TP53, RNA, 20(6):765-72, 2014

12. Ji D, Chen Z, Li M, Zhan T, Yao Y, Zhang Z, Xi J, Yan L, Gu J, MicroRNA-181a promotes tumor growth and liver metastasis in colorectal cancer by targeting the tumor suppressor WIF-1 Molecular Cancer 13:86, 2014

13. Yi Zhang, Lu Zhang, Jiashu Sun, Yulei Liu, Xingjie Ma, Shangjin Cui, Liying Ma, Jianzhong Jeff Xi, Xingyu Jiang. Point-of-Care Multiplexed Assays of Nucleic Acids Using Microcapillary-based Loop-Mediated Isothermal Amplification, Analytical Chemistry 86: 7057-7062, 2014

14. Ren X, Sun J, Housden B.E, Hu Y, Roesel C, Lin S, Liu L-P, Yang Z, Mao D, Sun L,  Wu Q, Ji J-Y, Xi J, Mohr S, Xu J, Perrimon N, Ni J-Q, Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9, Proceedings of the National Academy of Sciences 110:47 19012-19017, 2013

15. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi J, Qiu J-L, Gao C*, Targeted Genome Modification of Crop Plants Using the CRISPR/Cas System, Nature Biotech. 31, 686, 2013

16. Zhang H, Wu PY, Ma M, Ye Y, Hao Y, Yang J, Yin S, Sun C, Phan JH, Wang MD*, Xi J *. An integrative approach for the large-scale identification of human genome kinases regulating cancer metastasis, Nanomedicine 9(6):732-6, 2013

17. Chen J, Ma M, Shen N, Xi J*, Tian W*. Integration of cancer gene co-expression network and metabolic network to uncover potential cancer drug targets, J Proteome Res. 12(6):2354-64, 2013

18. Zhang Y, Qiao L, Ren Y, Wang X, Gao M, Tang Y, Xi J, Fu T-M, Jiang X, Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays, Biomicrofluidics 7, 034110, 2013

19. Chang N, Sun C, Gao L, Zhu D, Xu X, Zhu X, Xiong J-X*, and Xi J*. Genome Editing via the RNA-guided Cas9 Nuclease in Zebrafish Embryos, Cell Research, 23(4):465-72, 2013

20. Rong Y, Liu M, Ma L, Du W, Zhang H, Tian Y, Li Y, Ren H, Zhang C, Li L, Chen S, Xi J* & Li Yu*, Clathrin and phosphatidylinositol 4,5-bisphosphate regulate autophagic lysosome reformation, Nature Cell Biology 14(9):924-34, 2012

21. Zhao Wang, Juan Li, Gancheng Wang, Mingjun Jiang, Shenyi Yin, Changhong Sun, Hanshuo Zhang, Fengfeng Zhuang* and Jianzhong Jeff Xi*, An Integrated Chip for the High-throughput Synthesis of Transcription-Activator-like Effectors, Angew. Chem. Int. Ed., 124:34, 8633-8636, 2012

22. Ming Xu, Hao-Di Wu, Rong-Chang Li, Hai-Bo Zhang, Meng Wang, Jin Tao, Xin-Heng Feng, Yun-Bo Guo, Su-Fang Li, Shao-Ting Lai, Peng Zhou, Lin-Lin Li, Hua-Qian Yang, Guan-Zheng Luo, Yan Bai, Jianzhong J. Xi, Wei Gao, Qi-De Han, You-Yi Zhang, Xiu-Jie Wang, Xu Meng*, Shi-Qiang Wang*. Mir-24 Regulates Junctophilin-2 Expression in Cardiomyocytes, Circulation Research, 111: 837-841, 2012

23. Ping Yin, Dong Deng, Chuangye Yan, Xiaojing Pan, Jianzhong Jeff Xi, Nieng Yan, and Yigong Shi*. Specific DNA-RNA Hybrid Recognition by TAL Effectors, Cell Reports, 2(4):707-13, 2012

24. Yi Zhang, Xuwei Wang, Lusheng Song, Chuanlai Xu, Liying Ma, Zhanhua Li, Jianzhong Xi* and Xingyu Jiang*. Matrix-localization for fast analysis of arrayed microfluidic immunoassays, Anal. Methods, 4, 3466, 2012

25. Zhao Wang, Huang Huang, Hanshuo Zhang, Chang Hong Sun, Yang Hao, Junyu Yang, Yu Fan, and Jianzhong Xi*, A Magnetic Bead-Integrated Chip for the Large Scale Manufacture of Normalized esiRNAs, PLoS ONE, 7(6): e39419, 2012

26. Junyu Yang, Yang Hao, Jianzhong Jeff Xi*. Therapeutic Application of MicroRNAs against Human Cancers, J Lab Autom., Feb;18(1):30-3, 2012

27. Hanshuo Zhang, Yang Hao, Junyu Yang, Ying Zhuo, Juan Li, Changhong Sun, Shenyi Yin, Yanyi Huang and Jianzhong Xi*, Genome-wide Functional Screening of miR-23b as a Pleiotropic Modulator Suppressing Cancer Metastasis, Nature Communications, 2:554, 2011

28. Huang Huang, Qing Chang, Changhong Sun, Shenyi Yin, Juan Li and Jianzhong Xi*, A polyacrylamide microbead-integrated chip for the large-scale manufacture of ready-to-use esiRNA, Lab on a Chip, 11(6): 1036-1040, 2011

29. Huiping Yuan, Xiaoyong Zhang, Xiuqing Huang, Yonggang Lu, Weiqing Tang, Yong Man, Shu Wang, Jianzhong Xi* and Jian Li*, NADPH Oxidase 2-Derived Reactive Oxygen Species Mediate FFAs-Induced Dysfunction and Apoptosis of b-Cells via JNK, p38 MAPK and p53 Pathways, PLoS ONE, 5(12): e15726, 2011

30. Yu Z, Zhu Y, Zhang Y, Li J, Fang Q, Xi J, Yao B*. Nanoliter droplet array for microRNA detection based on enzymatic stem-loop probes ligation and SYBR Green real-time PCR, Talanta, 85(4):1760-5, 2011 

31. Smith AH, Robinson EM, Zhang XQ, Chow EK, Lin Y, Osawa E, Xi J and Ho D. Triggered release of therapeutic antibodies from nanodiamond complexes, Nanoscale, 3(7):2844-8, 2011

科研项目